Table 1 Summary of fixation methods and their effects on cilia marker immunolabeling
From: Fixation methods can differentially affect ciliary protein immunolabeling
| Antibodies | Cilia staining | ||||||
|---|---|---|---|---|---|---|---|
| PFA-S | PFA/PBS | MeOH | CBPFA | CB MeOH | CB CBPFA | CB CBPFA MeOH | |
| IMCD3 | |||||||
| β-tubulin | + | + | + | + | ++ | +++ | +++ |
| Acetylated-α- tubulin | +++ | +++ | ++ | +++ | +++ | +++ | +++ |
| Detyrosinated tubulin | +++ | +++ | +++ | + | + | +++ | +++ |
| Polyglutamylated tubulin | + | + | +/− | +++ | +++ | +++ | +++ |
| AC3 | + | + | − | − | − | − | − |
| Arl13b | +++ | +++ | + | +++ | − | − | − |
| CSPP1 | +/− | +/− | – | ++ | – | + | +++ |
| IFT20 | − | − | − | − | + | + | + |
| RPE | |||||||
| β-tubulin | +/− | + | + | + | ++ | +++ | +++ |
| Acetylated-α-tubulin | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| Detyrosinated tubulin | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
| Polyglutamylated tubulin | ++ | +++ | +/− | ++ | +++ | +/− | +++ |
| AC3 | − | − | − | − | − | − | − |
| Arl13b | +++ | +++ | − | +++ | − | − | − |
| CSPP1 | + | + | − | +++ | − | +++ | + |
| IFT20 | − | − | − | +/− | + | ++ | +++ |