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Table 1 Known and candidate Giardia basal body proteins including supporting evidence

From: Eight unique basal bodies in the multi-flagellated diplomonad Giardia lamblia

GiardiaDB Protein name Protein family PFAM Basal body and/or other subcellular localization Evidence
GL50803_6744 Centrin Centrin PF13499; PF13833 All basal bodies HOM, IFA
GL50803_104685 Caltractin Caltractin PF13499 All basal bodies HOM, IFA, GFP
GL50803_114218 Gamma-tubulin Tubulin PF00091; PF03953 All basal bodies, cytoplasm HOM, PRO, IFA, GFP
GL50803_5462 Delta-tubulin Tubulin PF00091 Cytoplasm HOM, GFP
GL50803_6336 Epsilon-tubulin Tubulin PF00091 Cytoplasm HOM, GFP
GL50803_17429 GCP2 Spc97 Spc98 PF04130 n.d. HOM
GL50803_12057 GCP3 Spc97_Spc98 PF04130 All basal bodies, median body, cytoplasmic caudal, posteriolateral, and anterior axonemes HOM, GFP
GL50803_104150 Kinase, PLK Ser/Thr kinase domain, POLO box domain PF00069, PF00659 All basal bodies EPI
GL50803_4689 Hypothetical protein None None All basal bodies PRO, EPI
GL50803_4692 Hypothetical protein None None All basal bodies PRO, EPI
GL50803_41512 Flagella associated protein Rib72 DUF1126 domain of unknown function PF06565 All basal bodies, cytoplasm PRO, EPI
GL50803_114546 Hypothetical protein None None All basal bodies, all cytoplasmic axonemes GFP
GL50803_3582 DUF390 domain containing protein DUF390 domain of unknown function PF04094 All basal bodies, all cytoplasmic axonemes GFP
GL50803_8974 Hypothetical protein None None All basal bodies, all cytoplasmic axonemes, median body, ciliary pocket GFP
GL50803_16935 Hypothetical protein None None All basal bodies, all flagella, ventral disc lateral crest, median body GFP
GL50803_15218 WD-40 repeat protein WD40 repeats PF00400 All basal bodies, all flagella, ventral disc lateral crest, median body PRO, IFA, GFP
GL50803_9117 CAMP-dependent protein kinase regulatory chain Cyclic nucleotide binding domain PF00027 All basal bodies, anterior axonemes, caudal axonemes EPI
GL50803_11214 Kinase, AGC PKA Kinase, AGC PKA PF00069 All basal bodies, anterior axonemes, caudal axonemes EPI
GL50803_16202 Axoneme central apparatus protein PF16/SPAG6 Central pair associated protein PF00514 All basal bodies, anterior axonemes, posteriorlateral axonemes, caudal axonemes, median body PRO, IFA, EPI
GL50803_11867 RIB43A RIB43A PF05914 All basal bodies, anterior axonemes, posteriorlateral axonemes, median body EPI
GL50803_7351 Hypothetical protein None None All basal bodies, ciliary pocket, cytoplasmic ventral, caudal, posteriolateral, and anterior axonemes GFP
GL50803_24412 Ankyrin-repeat domain containing protein Ankyrin-repeat domains PF12796 All basal bodies, cytoplasmic anterior and posteriolateral axonemes GFP
GL50803_10460 Hypothetical protein None None All basal bodies, cytoplasmic anterior axonemes, median body GFP
GL50803_17586 Ankyrin-repeat domain containing protein Ankyrin-repeat domains PF12796 All basal bodies, cytoplasmic anterior, posteriolateral and ventral axonemes GFP
GL50803_7192 FWWh domain containing protein FWWh domain of unknown function PF14922 All basal bodies, cytoplasmic caudal axonemes, posteriolateral and anterior axonemes GFP
GL50803_8557 Hypothetical protein None None All basal bodies, cytoplasmic caudal axonemes, posteriolateral and anterior axonemes GFP
GL50803_102455 GiKIN6a Kinesin-6 PF00225 All basal bodies, cytoplasmic posteriolateral and anterior axonemes, median body, ventral disc GFP
GL50803_11775 Kinase, ankyrin-repeat domain containing protein Ser/Thr kinase domain, ankyrin-repeat domains PF00069, PF1279 All basal bodies, cytoplasmic posteriolateral and caudal axonemes GFP
GL50803_15219 Macoilin domain containing protein Macoilin PF09726 All basal bodies, cytoplasmic posteriolateral axonemes, cytoplasm GFP
GL50803_15446 Hypothetical protein None None All basal bodies, cytoplasmic posteriolateral axonemes, median body GFP
GL50803_6709 Hypothetical protein None None All basal bodies, median body, all flagella, ventral disc lateral crest GFP
GL50803_92498 Kinase, NEK Kinase, NEK PF00069 All basal bodies, median body, anterior axonemes, posteriorlateral axonemes, caudal axonemes, ventral disc EPI
GL50803_17154 RRP7 domain containing protein Ribosomal RNA-processing protein 7 (RRP7) domain PF12923 All basal bodies, median body, cytoplasmic anterior axonemes, caudal axonemes, posteriolateral axonemes GFP
GL50803_4624 DUF4490 domain containing protein DUF4490 PF14892 All basal bodies, median body, cytoplasmic caudal, ventral, posteriolateral and anterior axonemes GFP
GL50803_13352 T-complex protein-10/Sas-4/CENPJ T complex protein 10 family C terminal domain PF07202 All basal bodies, median body, cytoplasmic posteriolateral axonemes, ciliary pocket PRO, IFA, GFP
GL50803_10232 LRR repeat domain containing protein CENP-F_leu_zip leucine-rich repeats PF10473 All basal bodies, median body, ventral disc GFP
GL50803_31671 RPB7 SHS2 domain containing protein N terminal SHS2 domain of RNA pol II subunit RPB7 PF03876 All basal bodies, nuclei, cytoplasmic posteriolateral and anterior axonemes GFP
GL50803_16220 Ankyrin-repeat domain containing protein Ankyrin-repeat domains PF12796 All basal bodies, plasma membrane, cytoplasm GFP
GL50803_16973 Hypothetical protein None None All basal bodies, posteriorlateral axonemes, median body PRO, IFA
GL50803_17096 Ankyrin-repeat domain containing protein Ankyrin-repeat domains PF12796 All basal bodies, ventral disc lateral crest GFP
GL50803_16424 MiflIP domain containing protein Mlf1IP PF10248 All basal bodies, ventral disc lateral crest, cytoplasmic anterior axonemes GFP
GL50803_17097 Ankyrin-repeat domain containing protein Ankyrin-repeat domains PF12796 All basal bodies, ventral disc lateral crest, cytoplasmic anterior axonemes, median body GFP
GL50803_101326 Hypothetical protein None None All basal bodies, ventral disc lateral crest, cytoplasmic caudal axonemes GFP
GL50803_15499 DUF390 domain containing protein DUF390 domain of unknown function PF04094 All basal bodies, ventral disc lateral crest, cytoplasmic posteriolateral axonemes GFP
GL50803_5568 DUF866 domain containing protein DUF866 PF05907 All basal bodies, ventral disc lateral crest, ventral axonemes GFP
GL50803_5010 Ser/Thr phosphatase PP2A-2 catalytic subunit Metallo-dependent phosphatases PF00149 All basal bodies, ventral disc, anterior axonemes, posteriorlateral axonemes, caudal axonemes PRO, IFA
GL50803_17563 Kinase, CMGC MAPK Kinase, CMGC MAPK PF00069 All basal bodies, ventral disc, caudal axonemes, median body EPI
GL50803_16279 Kinase, NEK Kinase, NEK PF00069 All basal bodies, ventral disc, caudal axonemes, posteriorlateral axonemes EPI
GL50803_5358 Aurora kinase Aurora kinase PF00069 All basal bodies, ventral disc, nuclei, anterior axonemes, median body EPI
GL50803_15193 Hypothetical protein None None Some basal bodies PRO, IFA, GFP
GL50803_29796 Hypothetical protein None None Some basal bodies, anterior flagella, ventral flagella, median body GFP
GL50803_6254 Hypothetical protein None None Some basal bodies, cytoplasmic posteriolateral axonomes GFP
GL50803_15455 PACRG1 Parkin coregulated protein PF10274 Cytoplasm, nuclei HOM, GFP
GL50803_14048 EB1 EBl-like C terminal motif PF03271 Nuclear membrane, median body GFP
GL50803_15956 FAP52 WD40 repeats PF00400 Plasma membrane, all axonemes HOM, GFP
GL50803_5333 Calmodulin EF-hand domain PF13499 n.d. HOM
GL50803_33762 POC1 WD repeat protein PF00400 n.d. HOM
GL50803_32375 POC18 None None n.d. HOM
GL50803_13372 FAP45 TPH domain PF13868 n.d. HOM
GL50803_5167 VFL3 None None n.d. HOM
GL50803_15248 Bub2 TBC domain PF00566 n.d. HOM
GL50803_8738 BBS1 BBS1 PF14779 n.d. HOM
GL50803_23934 BBS2 None None n.d. HOM
GL50803_10529 BBS4 TPR_1 Tetratricopeptide repeat PF01515 n.d. HOM
GL50803_8146 BBS5 DUF1448 domain of unknown function PF07289 n.d. HOM
GL50803_8508 BBS8 TPR_1 Tetratricopeptide repeat PF01515 n.d. HOM
  1. Evidence for candidate basal body proteins is based on homology to known basal body proteins in other organisms (HOM) [9, 41, 42], enrichments of Giardia basal body proteins in proteomic analyses (PRO) [19, 41] and/or subcellular localization using either heterologous antibodies (IFA) [25–27, 43, 44] or epitope-tagging (EPI) [19, 41]. Candidate basal body proteins identified with an ongoing, publically available, Giardia C-terminal GFP-tagging project for EuPATHDB [45] in our laboratory are also presented (GFP)
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Cilia

ISSN: 2046-2530

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